Exfection™ plasmid LE (Low Endotoxin) and EF(Endotoxin-Free) provide simple and fast method for the purification of plasmid DNA with low endotoxin contaminants.Endotoxins (also known as lipopolysaccharides, LPS) are present in the cell membrane of gram-negative bacteria, such as Escherichia coli. It is a common contaminant in plasmid preparations and can significantly reduce transfection efficiencies, if not removed during DNA preparations. These kits use two methods for the removal of endotoxins: advanced phase
Catalogue No. | Description | Pack Size | Price | Qty |
|
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GA-111-102 | GeneAll Exfection Plasmid LE | 200 mini preps | £164.00 | Quantity | Add to Order |
GA-111-110 | GeneAll Exfection Plasmid LE | 10 prep sample (1 per customer) | £0.00 | Quantity | Add to Order |
GA-111-150 | GeneAll Exfection Plasmid LE | 50 mini preps | £75.00 | Quantity | Add to Order |
GA-111-201 | GeneAll Exfection Plasmid LE | 100 midi prep | £640.00 | Quantity | Add to Order |
GA-111-226 | GeneAll Exfection Plasmid LE | 26 midi preps | £204.00 | Quantity | Add to Order |
GA-111-701 | Spin Column for Exfection LE/EF Midi | 25 each | £80.00 | Quantity | Add to Order |
GA-111-901 | Buffer P1 | 60 ml | £19.60 | Quantity | Add to Order |
GA-111-903 | Buffer P1 | 100 ml | £22.00 | Quantity | Add to Order |
GA-111-911 | Buffer P2 | 60 ml | £20.80 | Quantity | Add to Order |
GA-111-913 | Buffer P2 | 100 ml | £23.20 | Quantity | Add to Order |
GA-111-921 | Buffer G3 | 90 ml | £32.80 | Quantity | Add to Order |
GA-111-922 | Buffer G3 | 110 ml | £38.80 | Quantity | Add to Order |
GA-111-933 | Buffer EW1 | 150ml | £40.00 | Quantity | Add to Order |
GA-111-935 | Buffer EW1 | 300 ml | £63.00 | Quantity | Add to Order |
GA-111-944 | Buffer EW2 | 250 ml | £8.00 | Quantity | Add to Order |
GA-111-946 | Buffer EW2 | 400 ml | £13.00 | Quantity | Add to Order |
GA-111-952 | Buffer EF | 60 ml | £17.20 | Quantity | Add to Order |
GA-111-991 | Spin Column for Exfection LE mini | 100 each | £40.00 | Quantity | Add to Order |
GA-111-992 | Spin Column for Exfection LE mini | 200 each | £66.00 | Quantity | Add to Order |
Exfection™ plasmid LE (Low Endotoxin) and EF(Endotoxin-Free) provide simple and fast method for the purification of plasmid DNA with low endotoxin contaminants.Endotoxins (also known as lipopolysaccharides, LPS) are present in the cell membrane of gram-negative bacteria, such as Escherichia coli. It is a common contaminant in plasmid preparations and can significantly reduce transfection efficiencies, if not removed during DNA preparations. These kits use two methods for the removal of endotoxins: advanced phase
Description
ExfectionTM plasmid LE (Low Endotoxin) and EF(Endotoxin-Free) provide simple and fast method for thepurification of plasmid DNA with low endotoxin contaminants.Endotoxins (also known as lipopolysaccharides, LPS) are present in the cell membrane of gram-negative bacteria, such as Escherichia coli.It is a common contaminant in plasmid preparations and can significantly reduce transfection efficiencies, if not removed during DNA preparations. These kits use two methods for the removal of endotoxins: advanced phase separation and endotoxin removal washing. Endotoxin levels can be reduced to 0.1 EU/ug with ExfectionTM EF and to 10 EU/ug with ExfectionTM LE. Prepared plasmid DNA can be used for the transfection of most of cell lines in addition to most of molecular biological applications.
Features and Benefits
¦ Spin column format based on glassfiber membrane¦ Convenient clearing of lysate with EzClearTM filter (Midi)¦ High plasmid recoveries with high purity¦ Fast preparation time and simple procedure¦ High transfection efficiency in most cell-lines¦ No need of additional materials¦ No use of organic solvents
Procedure
Specification
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Exfection™ plasmid LE (Low Endotoxin) and EF(Endotoxin-Free) provide simple and fast method for the purification of plasmid DNA with low endotoxin contaminants.Endotoxins (also known as lipopolysaccharides, LPS) are present in the cell membrane of gram-negative bacteria, such as Escherichia coli. It is a common contaminant in plasmid preparations and can significantly reduce transfection efficiencies, if not removed during DNA preparations. These kits use two methods for the removal of endotoxins: advanced phase
Click here fro protocol
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Exfection™ plasmid LE (Low Endotoxin) and EF(Endotoxin-Free) provide simple and fast method for the purification of plasmid DNA with low endotoxin contaminants.Endotoxins (also known as lipopolysaccharides, LPS) are present in the cell membrane of gram-negative bacteria, such as Escherichia coli. It is a common contaminant in plasmid preparations and can significantly reduce transfection efficiencies, if not removed during DNA preparations. These kits use two methods for the removal of endotoxins: advanced phase
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If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
多种天然提取的人类蛋白质产品;多种兔抗人类蛋白质抗血清。
Albumin, Human Plasma | 16-16-011202 |
|
16-16-010700 |
Alpha 1 Antichymotrypsin, Human Plasma | 16-16-012400 |
Alpha 1 Antitrypsin, Human Plasma | 16-16-011609 |
Alpha 2 Antiplasmin, Human Plasma | 16-16-012901 |
Alpha 2 HS Glycoprotein, Human Plasma | 16-16-081907 |
Alpha 2 Macroglobulin, Human Plasma | 16-16-012013 |
Angiotensinogen, Human Plasma | 16-16-011407 |
Antisera to Albumin, Human Plasma | 01-16-011202 |
Antisera to Amylase, Human Pancreas | 01-19-010000 |
Antisera to Apolipoprotein AI, Human Plasma | 01-16-120101 |
Antisera to Apolipoprotein CII, Human Plasma | 01-16-120302 |
Antisera to Azurocidin, Human Neutrophil | 01-14-012621 |
Antisera to Catalase, Human Erythrocyte | 01-05-030000 |
Antisera to Cathepsin B, Human Liver | 01-12-030102 |
Antisera to Cathepsin D, Human Liver | 01-12-030104 |
Antisera to Cathepsin H, Human Liver | 01-12-030108 |
Antisera to Cathepsin L, Human Liver | 01-12-030112 |
Antisera to Elastase, Human Neutrophil | 01-14-051200 |
Antisera to Fibrinogen, Human Plasma | 01-16-060902 |
Antisera to Kallikrein, Human Plasma | 01-16-110112 |
Antisera to Myeloperoxidase, Human Neutrophil | 01-14-130000 |
Antisera to Thrombospondin, Human Platelet | 01-20-201319 |
Antisera to Trypsin, Human Pancreas | 01-19-032000 |
Antithrombin III, Human Plasma | 16-16-012020 |
Apolipoprotein AI, Human Plasma | 16-16-120101 |
Apolipoprotein AII, Human Plasma | 16-16-120102 |
Apolipoprotein AIV, Human Plasma | 16-16-120104 |
Apolipoprotein B, Human Plasma | 16-16-120200 |
Apolipoprotein CI, Human Plasma | 16-16-120301 |
Apolipoprotein CII, Human Plasma | 16-16-120302 |
Apolipoprotein CIII, Human Plasma | 16-16-120303 |
Apolipoprotein E, Human Plasma | 16-16-120500 |
Apotransferrin, Dog Plasma | 16-16-A32001-dog |
Apotransferrin, Human Plasma | 16-16-A32001 |
Apotransferrin, Human Plasma, Low Endotoxin Level | 16-16-A32001-LEL |
Apotransferrin, Mouse Plasma | 16-16-A32001-mouse |
Apotransferrin, Rat Serum | 16-16-A32001-rat |
Azurocidin, Human Neutrophil (Cationic protein 37) | 16-14-012621 |
Bacterial/Permeability-Increasing Protein, Human Neutrophil (BPI, CAP57) | 16-14-021609 |
C1 Esterase Inhibitor, Human Plasma | 16-16-031509 |
Calmodulin, Bovine Brain, High Purity | 16-BR-030112-HP-bovine |
Calpain-1, Human Erythrocyte | 16-05-030112 |
Calpain-1, Porcine Erythrocytes | 16-05-030112-P |
Catalase, Human Erythrocyte | 16-05-030000 |
Cathepsin B, Bovine Spleen | 16-SP-030102-bovine |
Cathepsin B, Human Liver | 16-12-030102 |
Cathepsin D, Bovine Spleen | 16-SP-030104-bovine |
Cathepsin D, Human Liver | 16-12-030104 |
Cathepsin G, Human Neutrophil | 16-14-030107 |
Cathepsin H, Human Liver | 16-12-030108 |
Cathepsin L, Human Liver | 16-12-030112 |
Cathepsin S, Human Spleen | 16-18-030118 |
Ceruloplasmin, Human Plasma | 16-16-030518 |
Chylomicrons, Human Plasma | 12-16-030825 |
Chymotrypsin, Human Pancreas | 16-19-030820 |
Defensins, Alpha, Mixed from Human Neutrophils (HNP) | 16-14-081416 |
Dipeptidyl Peptidase IV, Human Placenta | 16-23-041606 |
Elastase, Human Neutrophil | 16-14-051200 |
Eosinophil Cationic Protein, Human Eosinophils (ECP) | 16-15-050316 |
Eosinophil Derived Neurotoxin, Human Eosinophils (EDN) | 16-15-050414 |
Eosinophil Peroxidase, Human Eosinophils (EPO) | 16-15-160518 |
Factor Va, Human Plasma | 16-16-060601 |
GC-Globulin, Human Plasma, Mixed Type (维D结合蛋白) | 16-16-070307 |
Haptoglobin, Human Plasma, Mixed Type | 16-16-080116 |
Hemopexin, Human Plasma | 16-16-080513 |
Immunoglobulin A, Human Plasma | 16-16-090701 |
Immunoglobulin A1, Human Myeloma Protein | 16-16-090701-1M |
Immunoglobulin A2, Human Myeloma Plasma | 16-16-090701-2M |
Immunoglobulin D, Human Myeloma Plasma | 16-16-090704-M |
Immunoglobulin D, Human Plasma | 16-16-090704 |
Immunoglobulin E, Human Myeloma Plasma | 16-16-090705-M |
Immunoglobulin E, Human Plasma | 16-16-090705 |
Immunoglobulin G, Dog Plasma | 16-16-090707-dog |
Immunoglobulin G, Human Plasma | 16-16-090707 |
Immunoglobulin G, Human Plasma, Fab Fragment | 16-16-090707-Fab |
Immunoglobulin G, Human Plasma, FC Fragment | 16-16-090707-FC |
Immunoglobulin G, Mouse Plasma | 16-16-090707-mouse |
Immunoglobulin G, Rat Serum | 16-16-090707-rat |
Immunoglobulin G1, Human Myeloma Plasma | 16-16-090707-1M |
Immunoglobulin G2, Human Myeloma Plasma | 16-16-090707-2M |
Immunoglobulin G3, Human Plasma | 16-16-090707-3 |
Immunoglobulin G4, Human Myeloma Plasma | 16-16-090707-4M |
Immunoglobulin M, Fc5mu, Human Plasma | 16-16-090713-Mfc5u |
Immunoglobulin M, Human Myeloma Plasma | 16-16-090713-M |
Immunoglobulin M, Human Plasma | 16-16-090713 |
Immunoglobulin M, mu Chain, Human Plasma | 16-16-090713-MU |
Kallikrein, Human Plasma | 16-16-110112 |
Kininogen, HMW, Human Plasma | 16-16-110914-H |
Kininogen, LMW, Human Plasma | 16-16-110914-L |
Lactalbumin, Human Milk | 13-16-011202 |
Lactoferrin, Human Neutrophil | 16-14-120103 |
Lipoprotein a, [Lp(a)], Human Plasma | 12-16-121601 |
Lipoproteins, High Density, Human Plasma | 12-16-080412 |
Lipoproteins, High Density, Human Plasma, 适于细胞培养 | 12-16-080412-TC |
Lipoproteins, Intermediate Density, Human Plasma | 12-16-090412 |
Lipoproteins, Low Density, Human Plasma | 12-16-120412 |
Lipoproteins, Low Density, Human Plasma, 适于细胞培养 | 12-16-120412-TC |
Lipoproteins, Very Low Density, Human Plasma | 12-16-221204 |
Lipoproteins, Very Low Density, Human Plasma, 适于细胞培养 | 12-16-221204-TC |
Lysozyme, Human Neutrophil | 16-14-122519 |
Myeloperoxidase, Human Neutrophil | 16-14-130000 |
Plasmin, Human Plasma, Frozen | 16-16-161213-F |
Plasmin, Human Plasma, Lyophilized | 16-16-161213-L |
Plasminogen, Human Plasma | 16-16-161200 |
Platelet Factor 4, Human Platelet | 16-20-060306 |
Prealbumin, Human Plasma (Transthyretin) | 16-16-161801 |
Proteinase 3, Human Neutrophil | 16-14-161820 |
S-100 Protein, Bovine Brain | 16-BR-190816-bovine |
S-100b Protein, Bovine Brain | 16-BR-190802-bovine |
S-100αα Protein, Bovine Brain | 16-BR-190801-bovine |
Serum Amyloid A, Human Plasma | 16-16-190101 |
Thrombospondin, Human Platelet | 16-20-201319 |
Transferrin, Dog Plasma | 16-16-032001-dog |
Transferrin, Human Plasma | 16-16-032001 |
Transferrin, Human Plasma, 组织培养级 | 16-16-032001-LEL |
Transferrin, Mouse Plasma | 16-16-032001-mouse |
Transferrin, Rat Serum | 16-16-032001-rat |
Trypsin, Human Pancreas | 16-19-032000 |
Vitronectin, Human Plasma | 16-16-220920 |