Transfection - EZ Biosystems: Cell Lines

Cell LineInformation:

Designations: DU 145 Organism: Homo sapiens (human) Tissue: prostate carcinoma; derived from metastatic site: brain Age: 69 years Gender: male Ethnicity: Caucasian Morphology: epithelial Growth properties: adherent Tumorigenic: Yes, in nude mice; forms adenocarcinoma (grade II) consistent with prostatic primary Antigen Expression: Blood Type O; RhDepositor: K.R. Stone DU 145 was isolated by K.R. Stone et al. from a lesion in the brain of a patient with metastatic carcinoma of the prostate and a 3 year history of lymphocytic leukemia. Ultrastructural analyses of both the cell line and original tumor revealed microvilli, tonofilaments and desmosomes, many mitochondria, well developed Golgi and heterogenous lysosomes. The cells do not express prostate antigen.

DU 145 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

The Transfection Reagent:

DU 145 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on DU 145 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for DU 145 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of DU 145 Cell Avalanche™ Transfection Reagent for transfection on DU 145 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into DU 145 Cells
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Simplest and straightforward protocols
• Compatible with transfection in any plate formats
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on PC-3 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for PC-3 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested on DU 145 cells by tranfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as DU 145 Cell Avalanche Transfection Reagent. FIG. 2. DU 145 cells were transfected with GFP vector (pEGFP-N3) by using DU 145 Cell Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.

For Other Cells:

DU 145 Cell Avalanche™ Transfection Reagent (human prostate carcinoma cell) can also be used on the following cells with high transfection efficiencies. 22Rv1 Cell VCaP Cell PC-3 Cell Vero Cell 293 Cell 293T/17 Cell Calu-3 Cell Caki-1 Cell MDCK Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

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