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℡ 4000-520-616
Athens Research/Cambio - Excellence in Molecular Biology/0.25 grams/10-3151-02
产品编号:10-3151-02
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场      地:美国(厂家直采)
产品分类: 蛋白类>多肽>多肽合成>
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Athens Research/Cambio - Excellence in Molecular Biology/0.25 grams/10-3151-02
商品介绍
Oligo Synthesis

Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.

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2"-OMe-Ac-C-PACE Phosphoramidite

2"-OMe-Ac-C-PACE Phosphoramidite

Glen Research

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10-3151-022"-OMe-Ac-C-PACE Phosphoramidite0.25 grams£88.00£83.60Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView OfferQuantityAdd to Order

2"-OMe-Ac-C-PACE Phosphoramidite

2"-OMe-Ac-C-PACE Phosphoramidite

Glen Research

2"-OMe-A-PACE Phosphoramidite
PACE modifications have enjoyed a resurgence in interest as applied to the field of CRISPR gene editing. In an initial publication, it was shown that single guide RNAs (sgRNA) provided significantly higher activity in cells when 2"-O-methylthiophosphonoacetates were incorporated on the ends of the guide RNA to protect against cellular nucleases.1 In subsequent studies, 2"-OMe PACE modified sgRNAs were also shown to significantly increase on-target specificity of the CRISPR-Cas9 DNA cleavage in eukaryotic cells. In a recent paper, the incorporation of 2"-OMe PACE modified nucleotides in the 20-nucleotide guide region of the sgRNA was shown to decrease off-target cutting by over an order of magnitude while in most cases increasing the overall on-target efficiency as compared to unmodified single guide RNA.2
As an optimal cycle, we recommend using DCI as an activator (30-3150-XX) and a 15 minute coupling time. Following coupling, cap using Unicap with a regular coupling time and then oxidize using 0.5 M CSO for 3 minutes. Alternatively, a 33 minute coupling time using 0.45 M tetrazole, oxidation using low-water iodine (40-4032-XX) followed by capping with 6.5% DMAP as Cap B will give acceptable results. For deprotection, pre-treat the synthesis column with 1.5% DBU in anhydrous acetonitrile for 60 minutes at room temperature to remove 1,1-dimethyl-2-cyanoethyl protecting groups. Rinse the column with ACN, dry under argon and complete deprotection with 40% Methylamine for 2 hours at room temperature.
Formula: C46H58N5O10P
M.W.: 871.97
F.W.: 361.25

Diluent: Anhydrous Acetonitrile

Coupling: 15 minute coupling time using 0.25M DCI (30-3150-xx) is recommended. Using the phosphoramidite-based capping reagent Unicap (10-4410-xx), cap using a regular coupling time and then oxidize with 0.5M CSO for 3 minutes for best results.

Deprotection: Pretreat synthesis column with 1.5% DBU in anhydrous acetonitrile for 60 minutes at room temperature. Rinse with acetonitrile and dry with argon. Deprotect using 40% methylamine for 2 hours at room temperature.

Storage: Freezer storage, -10 to -30°C, dry

Stability in Solution: 2-3 days

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No.PackSizeGrams/Pack0.1M Dil.(mL)LV40LV20040nm0.2µm1µm10µm
Approximate Number of Additions
Expedite
Cat.No.PackSizeGrams/PackDilution(mL)Molarity50nm0.2µm1µm15µm
Approximate Number of Additions

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

2"-OMe-Ac-C-PACE Phosphoramidite

2"-OMe-Ac-C-PACE Phosphoramidite

Glen Research

SDS

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

2"-OMe-Ac-C-PACE Phosphoramidite

2"-OMe-Ac-C-PACE Phosphoramidite

Glen Research

Glen Report 30.1: Significant Improvement of CRISPR Specificity with 2"-OMe-PACE Modifications

Glen Report 30.1: Use of 2"-OMe-PACE Monomers During Oligo Synthesis

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

品牌介绍

多种天然提取的人类蛋白质产品;多种兔抗人类蛋白质抗血清。

Albumin, Human Plasma 16-16-011202
 
 
Alpha 1 Acid Glycoprotein, Human Plasma
16-16-010700
Alpha 1 Antichymotrypsin, Human Plasma 16-16-012400
Alpha 1 Antitrypsin, Human Plasma 16-16-011609
Alpha 2 Antiplasmin, Human Plasma 16-16-012901
Alpha 2 HS Glycoprotein, Human Plasma 16-16-081907
Alpha 2 Macroglobulin, Human Plasma 16-16-012013
Angiotensinogen, Human Plasma 16-16-011407
Antisera to Albumin, Human Plasma 01-16-011202
Antisera to Amylase, Human Pancreas 01-19-010000
Antisera to Apolipoprotein AI, Human Plasma 01-16-120101
Antisera to Apolipoprotein CII, Human Plasma 01-16-120302
Antisera to Azurocidin, Human Neutrophil 01-14-012621
Antisera to Catalase, Human Erythrocyte 01-05-030000
Antisera to Cathepsin B, Human Liver 01-12-030102
Antisera to Cathepsin D, Human Liver 01-12-030104
Antisera to Cathepsin H, Human Liver 01-12-030108
Antisera to Cathepsin L, Human Liver 01-12-030112
Antisera to Elastase, Human Neutrophil 01-14-051200
Antisera to Fibrinogen, Human Plasma 01-16-060902
Antisera to Kallikrein, Human Plasma 01-16-110112
Antisera to Myeloperoxidase, Human Neutrophil 01-14-130000
Antisera to Thrombospondin, Human Platelet 01-20-201319
Antisera to Trypsin, Human Pancreas 01-19-032000
Antithrombin III, Human Plasma 16-16-012020
Apolipoprotein AI, Human Plasma 16-16-120101
Apolipoprotein AII, Human Plasma 16-16-120102
Apolipoprotein AIV, Human Plasma 16-16-120104
Apolipoprotein B, Human Plasma 16-16-120200
Apolipoprotein CI, Human Plasma 16-16-120301
Apolipoprotein CII, Human Plasma 16-16-120302
Apolipoprotein CIII, Human Plasma 16-16-120303
Apolipoprotein E, Human Plasma 16-16-120500
Apotransferrin, Dog Plasma 16-16-A32001-dog
Apotransferrin, Human Plasma 16-16-A32001
Apotransferrin, Human Plasma, Low Endotoxin Level 16-16-A32001-LEL
Apotransferrin, Mouse Plasma 16-16-A32001-mouse
Apotransferrin, Rat Serum 16-16-A32001-rat
Azurocidin, Human Neutrophil (Cationic protein 37) 16-14-012621
Bacterial/Permeability-Increasing Protein, Human Neutrophil (BPI, CAP57) 16-14-021609
C1 Esterase Inhibitor, Human Plasma 16-16-031509
Calmodulin, Bovine Brain, High Purity 16-BR-030112-HP-bovine
Calpain-1, Human Erythrocyte 16-05-030112
Calpain-1, Porcine Erythrocytes 16-05-030112-P
Catalase, Human Erythrocyte 16-05-030000
Cathepsin B, Bovine Spleen 16-SP-030102-bovine
Cathepsin B, Human Liver 16-12-030102
Cathepsin D, Bovine Spleen 16-SP-030104-bovine
Cathepsin D, Human Liver 16-12-030104
Cathepsin G, Human Neutrophil 16-14-030107
Cathepsin H, Human Liver 16-12-030108
Cathepsin L, Human Liver 16-12-030112
Cathepsin S, Human Spleen 16-18-030118
Ceruloplasmin, Human Plasma 16-16-030518
Chylomicrons, Human Plasma 12-16-030825
Chymotrypsin, Human Pancreas 16-19-030820
Defensins, Alpha, Mixed from Human Neutrophils (HNP) 16-14-081416
Dipeptidyl Peptidase IV, Human Placenta 16-23-041606
Elastase, Human Neutrophil 16-14-051200
Eosinophil Cationic Protein, Human Eosinophils (ECP) 16-15-050316
Eosinophil Derived Neurotoxin, Human Eosinophils (EDN) 16-15-050414
Eosinophil Peroxidase, Human Eosinophils (EPO) 16-15-160518
Factor Va, Human Plasma 16-16-060601
GC-Globulin, Human Plasma, Mixed Type (维D结合蛋白) 16-16-070307
Haptoglobin, Human Plasma, Mixed Type 16-16-080116
Hemopexin, Human Plasma 16-16-080513
Immunoglobulin A, Human Plasma 16-16-090701
Immunoglobulin A1, Human Myeloma Protein 16-16-090701-1M
Immunoglobulin A2, Human Myeloma Plasma 16-16-090701-2M
Immunoglobulin D, Human Myeloma Plasma 16-16-090704-M
Immunoglobulin D, Human Plasma 16-16-090704
Immunoglobulin E, Human Myeloma Plasma 16-16-090705-M
Immunoglobulin E, Human Plasma 16-16-090705
Immunoglobulin G, Dog Plasma 16-16-090707-dog
Immunoglobulin G, Human Plasma 16-16-090707
Immunoglobulin G, Human Plasma, Fab Fragment 16-16-090707-Fab
Immunoglobulin G, Human Plasma, FC Fragment 16-16-090707-FC
Immunoglobulin G, Mouse Plasma 16-16-090707-mouse
Immunoglobulin G, Rat Serum 16-16-090707-rat
Immunoglobulin G1, Human Myeloma Plasma 16-16-090707-1M
Immunoglobulin G2, Human Myeloma Plasma 16-16-090707-2M
Immunoglobulin G3, Human Plasma 16-16-090707-3
Immunoglobulin G4, Human Myeloma Plasma 16-16-090707-4M
Immunoglobulin M, Fc5mu, Human Plasma 16-16-090713-Mfc5u
Immunoglobulin M, Human Myeloma Plasma 16-16-090713-M
Immunoglobulin M, Human Plasma 16-16-090713
Immunoglobulin M, mu Chain, Human Plasma 16-16-090713-MU
Kallikrein, Human Plasma 16-16-110112
Kininogen, HMW, Human Plasma 16-16-110914-H
Kininogen, LMW, Human Plasma 16-16-110914-L
Lactalbumin, Human Milk 13-16-011202
Lactoferrin, Human Neutrophil 16-14-120103
Lipoprotein a, [Lp(a)], Human Plasma 12-16-121601
Lipoproteins, High Density, Human Plasma 12-16-080412
Lipoproteins, High Density, Human Plasma, 适于细胞培养 12-16-080412-TC
Lipoproteins, Intermediate Density, Human Plasma 12-16-090412
Lipoproteins, Low Density, Human Plasma 12-16-120412
Lipoproteins, Low Density, Human Plasma, 适于细胞培养 12-16-120412-TC
Lipoproteins, Very Low Density, Human Plasma 12-16-221204
Lipoproteins, Very Low Density, Human Plasma, 适于细胞培养 12-16-221204-TC
Lysozyme, Human Neutrophil 16-14-122519
Myeloperoxidase, Human Neutrophil 16-14-130000
Plasmin, Human Plasma, Frozen 16-16-161213-F
Plasmin, Human Plasma, Lyophilized 16-16-161213-L
Plasminogen, Human Plasma 16-16-161200
Platelet Factor 4, Human Platelet 16-20-060306
Prealbumin, Human Plasma (Transthyretin) 16-16-161801
Proteinase 3, Human Neutrophil 16-14-161820
S-100 Protein, Bovine Brain 16-BR-190816-bovine
S-100b Protein, Bovine Brain 16-BR-190802-bovine
S-100αα Protein, Bovine Brain 16-BR-190801-bovine
Serum Amyloid A, Human Plasma 16-16-190101
Thrombospondin, Human Platelet 16-20-201319
Transferrin, Dog Plasma 16-16-032001-dog
Transferrin, Human Plasma 16-16-032001
Transferrin, Human Plasma, 组织培养级 16-16-032001-LEL
Transferrin, Mouse Plasma 16-16-032001-mouse
Transferrin, Rat Serum 16-16-032001-rat
Trypsin, Human Pancreas 16-19-032000
Vitronectin, Human Plasma 16-16-220920

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