Cell culture contamination control: Detection

Venor®GeM Advance

Venor®GeM Advance contains PCR reaction tubes pre-coated with all PCR reagents to reduce the total assay time without need to prepare aliquots of a master mix. For additional convenience the gel loading buffer and dye are already included in the reaction buffer. After thermal cycling the PCR can be loaded directly on the agarose gel.

Type of PCR

For conventional, endpoint PCR

Scope of Application

Applicable in research for direct testing of cell cultures and cell culture-derived biologicals. Intended for research use only. Not recommended for clinical diagnostics or testing of human samples.

Kit Components

Primer sets, nucleotides, polymerase and internal control DNA, lyophilized, filled in 0.2 ml PCR reaction tubes Rehydration buffer including gel loading buffer and running dye Positive controls, lyophilized, filled in 0.2 ml PCR reaction tubes PCR grade water

Package Sizes

• Cat. No. 11-7024 24 Tests

• Cat. No. 11-7048 48 Tests

• Cat. No. 11-7096 96 Tests

• Cat. No. 11-7240 240 Tests

Result evaluation

Gel electrophoresis at endpoint of PCRRequired Consumables

Agarose and DNA-specific dye for gel staining

Required lab devices

Regular block-heated PCR cycler Agarose gel electrophoresis and gel documentation system Pipetting equipment Tube centrifuge

Shelf Life and Storage

Kit components are maintainable at2 to8 °C for at least 6 months. After rehydratisation of the individual PCR tubes the reagents must be used within 1 hour.

EP 2.6.7 compliance

No

Fig. Amplified PCR products are visualized by standard gel electrophoresis.

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Venor^®GeM Advance protocol

Quick Visual protocol

Please note: all protocols off site are the responsibility of the products supplier

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Required consumables:

Agarose and DNA-specific dye for gel staining

Required lab devices:

Regular block-heated PCR cyclerAgarose gel electrophoresis and gel documentation systemPipetting equipmentTube centrifuge

Shelf life and storage:

Kit components are maintainable at2 to8 °C for at least 6 months. After rehydratisation of the individual PCR tubes the reagents must be used within 1 hour.

EP 2.6.7 compliance:

No

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Application

For the rapid and reliable detection of mycoplasma in various in situ biologicals including cell cultures and virus stocks

Benefits

• Sensitive - Detection of 5 copies/µl of mycoplasma DNA that corresponds to 10 mycoplasma genomes per sample volume.
• Specific - The kit is specific for more than 79 mycoplasma species including all relevant species mentioned in the Eur. Ph. 2.6.7. For the detection of the avian associated mycoplasma species M. synoviae and M. gallisepticum the product Venor®MGS is highly recommended. Eukaryotic and bacterial DNA is not amplified.
• Reliable - The tubes contain internal control DNA for individual inhibition control. The pre-aliquoted reagents optimize reproducibility and minimize operator-dependent variability of results.
• Simple - Only rehydration buffer, polymerase and sample have to be added to the PCR tube.
• Clear - A clear and easily interpretable result is obtained with one PCR reaction.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

• Venor™GeM; Frequently Asked Questions
• Troubleshooting: PCR & qPCR kits
• Venor™GeM Advance Protocol