PCR : PCR Products

Dependable, consistent high-fidelity PCR results with every target DNA template.

• Successful PCR, the first time and every time
• PCR amplification of difficult or high-GC templates (Fig. 1)
• Multiplex PCR compatible (Fig. 2)
• Robust amplification of targets up to 20 kb long
• High accuracy with 3-fold lower error rate than Taq DNA polymerase (error rate = 1 in 30,000)
• Extremely high sensitivity and specificity using the PCR Enhancer Technology (with Betaine)

The FailSafe™ PCR System provides dependable, consistent high-fidelity PCR results for every DNA template, regardless of its source or sequence. The FailSafe™ PCR System will faithfully amplify your template every time….We promise! Applications End-point PCR optimization Consistent and reproducible PCR How the FailSafe System Works First, purchase the FailSafe PCR PreMix Selection Kit (catalog number FS99060). The FailSafe PCR PreMix Selection Kit is composed of: FailSafe PCR Enzyme Blend (60 Units) Twelve (12) different FailSafe™ 2X PCR PreMixes. Each 2X PreMix contains dNTPs, Buffer and different concentrations of Mg and Epicentre"s proprietary PCR Enhancer (with Betaine). Prepare and perform PCR reactions using your template and PCR primers with each of the 12 FailSafe™ 2X PCR PreMixes and the FailSafe PCR Enzyme. Analyze, by agarose gel electrophoresis, the PCR product produced from each of the 12 reactions and visually determine which PreMix gives the best amplification (e.g., a single, strong PCR product of the desired length or "as expected" multiplex PCR results). We guarantee that one of the FailSafe PCR 2X PreMixes will give the desired amplification product. For reliable and consistent PCR results, use the FailSafe PCR Enzyme and the best 2X PreMix identified in the optimization experiments for all subsequent amplifications with that template/primer combination. To reduce costs, purchase a FailSafe PCR System (Cat. No. FS99100, FS99250,or FS9901K) which provides sufficient enzyme and the appropriate amount of your favorite 2X PCR Premix(es) instead of buying the enzyme and 2X PCR Premixes separately.

Figure 1. Amplification of an 80%-85% GC-rich region of the human fragile X gene. PCR was performed using the FailSafe™ PCR System, schematically depicted above. Lanes A-L show the amplification products resulting from PCR using the 12 FailSafe PCR PreMixes. Lane M, molecular weight marker. In this experiment, optimal amplification was obtained with FailSafe PCR PreMix J. The size of the expected amplicon is indicated by an arrow.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Protocols for: FailSafe™ PCR System

Due to the constant updating of the protocols by the manufacturer we have provided a direct link to Epicentre’s product page, where the latest protocol is available.

Please note this will open a new page or window on your computer.

Failsafe PCR PreMix Selection Kit / FailSafe™ PCR System / FailSafe™ Enzyme Mix Only / FailSafe™ PCR 2X PreMixes Protocol

(catalogue number FS99060 / FS99100 / FS99250 / FS9901K / FSE51100 / FSE5101K / FSP995A→L)

Please note: all protocols off site are the responsibility of the products supplier

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Customer endorsement

"EPICENTRE products are great, as for FailSafe™ PCR, we use it all the time."

Prof. Sir Martin Evans, FRSNobel Laureate, Cardiff University, Wales, UK

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118. Eskra, L., et al. (2001) Brucella abortus Genes Identified following Constitutive Growth and Macrophage Infection, Infect. Immun. 69 , 7736-7742.
119. Forteza, R., et al. (2001) Hyaluronan serves a novel role in airway mucosal host defense, FASEB J 15 , 2179-2186.
120. Pajor, A. M., et al. (2001) Cloning and functional characterization of a high-affinity Na /dicarboxylate cotransporter from mouse brain, Am J Physiol Cell Physiol 280 , C1215-1223.
121. Scholes, D. T., et al. (2001) Multiple Regulators of Ty1 Transposition in Saccharomyces cerevisiae Have Conserved Roles in Genome Maintenance, Genetics 159 , 1449-1465.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Contains dNTPs, buffer, and various amounts of MgCl₂ and FailSafe™ PCR Enhancer (with betaine*)

* Patents issued and pending on FailSafe™ PCR Enhancer (with betaine)

FailSafe™ PCR Premix Selection Kits: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser"s own internal research. No other patent rights (such as 5´ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Applications

• Simplified PCR optimization.
• PCR of difficult or high-GC templates (Fig. 1).
• Multiplex PCR (Fig. 2).
• PCR amplifications up to 20 kb for cloning

Benefits

• Successful PCR, the first time and every time.
• High PCR accuracy, using a unique PCR Enzyme Mix with a 3-fold lower error rate than Taq DNA polymerase.
• Extremely high sensitivity and specificity using the PCR Enhancer Technology.
• FailSafe PCR PreMixes contain all components needed for successful PCR.
• Generates PCR products suitable for both TA cloning and blunt-end cloning.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

• Failsafe™ Frequently Asked Questions
• Failsafe™ and related Epicentre Products