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℡ 4000-520-616
℡ 4000-520-616
Athens Research/Cambio - Excellence in Molecular Biology/1.5 ml/EZT-22RV-1-15
产品编号:EZT-22RV-1-15
市  场 价:¥0.00
场      地:美国(厂家直采)
产品分类: 蛋白类>多肽>多肽合成>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:待定
品      牌: Athens Research
公司分类:
Athens Research/Cambio - Excellence in Molecular Biology/1.5 ml/EZT-22RV-1-15
商品介绍
Transfection - EZ Biosystems

Transfection - EZ Biosystems: Cell Lines

Avalanche®-Cell Type / Cell Line-specific Transfection Reagent series:

Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines).Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated.

For primary cells click HERE

To learn why you should use Avalanche®Cell Type-Specific Transfection reagents, clickHERE; To learn how EZ Biosystems developed Avalanche®Cell Type-Specific Transection Reagents, clickHERE

  • Catalogue
  • Description

22Rv1 Cell Avalanche™ Transfection Reagent (human prostate carcinoma cell)

22Rv1 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting 22Rv1 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for 22Rv1 cells.

EZ Biosystems

Catalogue No.DescriptionPack SizePriceQty
  • Change to:
  • €
EZT-22RV-1-0522Rv1 Cell Transfection Reagent (human prostate carcinoma cell)0.5 ml£399.00QuantityAdd to Order
EZT-22RV-1-1522Rv1 Cell Transfection Reagent (human prostate carcinoma cell)1.5 ml£699.00QuantityAdd to Order

22Rv1 Cell Avalanche™ Transfection Reagent (human prostate carcinoma cell)

22Rv1 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting 22Rv1 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for 22Rv1 cells.

EZ Biosystems

Cell LineInformation:

Designations: 22Rv1 Organism: Homo sapiens (human) Tissue: carcinoma; prostate Morphology: epithelial Growth properties: adherent Tumorigenic: yes, forms tumors in nude mice Antigen expression: prostate specific antigen (PSA) DoublingTime: 40 hours Receptors expressed: androgen Depositors: J.W. Jacobberger 22Rv1 is a human prostate carcinoma epithelial cell line derived from a xenograft that was serially propagated in mice after castration-induced regression and relapse of the parental, androgen-dependent CWR22 xenograft. The cell line expresses prostate specific antigen. Growth is stimulated by epidermal growth factor (EGF) but is not inhibited by transforming growth factor beta-1 (TGF beta- 1).

The Transfection Reagent:

22Rv1 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting 22Rv1 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for 22Rv1 cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

  • Unique formulation-maximize transfection performance in 22Rv1 cells.
  • Highest efficiency to ensure experimental success
  • Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
  • Deliver single or multiple plasmids
  • Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
  • Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
  • Economical: High efficiency means less amount of nucleic acid & reagent is needed
  • Developed and manufactured by EZ Biosystems
Data:
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on PC-3 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for PC-3 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested on 22Rv1 cells by tranfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as 22Rv1 Cell Avalanche Transfection Reagent.
FIG. 2. 22Rv1 cells were transfected with GFP vector (pEGFP-N3) by using 22Rv1 Cell Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
For Other Cells:
22Rv1 Cell Avalanche™ Transfection Reagent (human prostate carcinoma cell) can also be used on the following cells with high transfection efficiencies.

DU 145 Cell VCaP Cell PC-3 Cell Vero Cell 293 Cell 293T/17 Cell Calu-3 Cell Caki-1 Cell MDCK Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

品牌介绍

多种天然提取的人类蛋白质产品;多种兔抗人类蛋白质抗血清。

Albumin, Human Plasma 16-16-011202
 
 
Alpha 1 Acid Glycoprotein, Human Plasma
16-16-010700
Alpha 1 Antichymotrypsin, Human Plasma 16-16-012400
Alpha 1 Antitrypsin, Human Plasma 16-16-011609
Alpha 2 Antiplasmin, Human Plasma 16-16-012901
Alpha 2 HS Glycoprotein, Human Plasma 16-16-081907
Alpha 2 Macroglobulin, Human Plasma 16-16-012013
Angiotensinogen, Human Plasma 16-16-011407
Antisera to Albumin, Human Plasma 01-16-011202
Antisera to Amylase, Human Pancreas 01-19-010000
Antisera to Apolipoprotein AI, Human Plasma 01-16-120101
Antisera to Apolipoprotein CII, Human Plasma 01-16-120302
Antisera to Azurocidin, Human Neutrophil 01-14-012621
Antisera to Catalase, Human Erythrocyte 01-05-030000
Antisera to Cathepsin B, Human Liver 01-12-030102
Antisera to Cathepsin D, Human Liver 01-12-030104
Antisera to Cathepsin H, Human Liver 01-12-030108
Antisera to Cathepsin L, Human Liver 01-12-030112
Antisera to Elastase, Human Neutrophil 01-14-051200
Antisera to Fibrinogen, Human Plasma 01-16-060902
Antisera to Kallikrein, Human Plasma 01-16-110112
Antisera to Myeloperoxidase, Human Neutrophil 01-14-130000
Antisera to Thrombospondin, Human Platelet 01-20-201319
Antisera to Trypsin, Human Pancreas 01-19-032000
Antithrombin III, Human Plasma 16-16-012020
Apolipoprotein AI, Human Plasma 16-16-120101
Apolipoprotein AII, Human Plasma 16-16-120102
Apolipoprotein AIV, Human Plasma 16-16-120104
Apolipoprotein B, Human Plasma 16-16-120200
Apolipoprotein CI, Human Plasma 16-16-120301
Apolipoprotein CII, Human Plasma 16-16-120302
Apolipoprotein CIII, Human Plasma 16-16-120303
Apolipoprotein E, Human Plasma 16-16-120500
Apotransferrin, Dog Plasma 16-16-A32001-dog
Apotransferrin, Human Plasma 16-16-A32001
Apotransferrin, Human Plasma, Low Endotoxin Level 16-16-A32001-LEL
Apotransferrin, Mouse Plasma 16-16-A32001-mouse
Apotransferrin, Rat Serum 16-16-A32001-rat
Azurocidin, Human Neutrophil (Cationic protein 37) 16-14-012621
Bacterial/Permeability-Increasing Protein, Human Neutrophil (BPI, CAP57) 16-14-021609
C1 Esterase Inhibitor, Human Plasma 16-16-031509
Calmodulin, Bovine Brain, High Purity 16-BR-030112-HP-bovine
Calpain-1, Human Erythrocyte 16-05-030112
Calpain-1, Porcine Erythrocytes 16-05-030112-P
Catalase, Human Erythrocyte 16-05-030000
Cathepsin B, Bovine Spleen 16-SP-030102-bovine
Cathepsin B, Human Liver 16-12-030102
Cathepsin D, Bovine Spleen 16-SP-030104-bovine
Cathepsin D, Human Liver 16-12-030104
Cathepsin G, Human Neutrophil 16-14-030107
Cathepsin H, Human Liver 16-12-030108
Cathepsin L, Human Liver 16-12-030112
Cathepsin S, Human Spleen 16-18-030118
Ceruloplasmin, Human Plasma 16-16-030518
Chylomicrons, Human Plasma 12-16-030825
Chymotrypsin, Human Pancreas 16-19-030820
Defensins, Alpha, Mixed from Human Neutrophils (HNP) 16-14-081416
Dipeptidyl Peptidase IV, Human Placenta 16-23-041606
Elastase, Human Neutrophil 16-14-051200
Eosinophil Cationic Protein, Human Eosinophils (ECP) 16-15-050316
Eosinophil Derived Neurotoxin, Human Eosinophils (EDN) 16-15-050414
Eosinophil Peroxidase, Human Eosinophils (EPO) 16-15-160518
Factor Va, Human Plasma 16-16-060601
GC-Globulin, Human Plasma, Mixed Type (维D结合蛋白) 16-16-070307
Haptoglobin, Human Plasma, Mixed Type 16-16-080116
Hemopexin, Human Plasma 16-16-080513
Immunoglobulin A, Human Plasma 16-16-090701
Immunoglobulin A1, Human Myeloma Protein 16-16-090701-1M
Immunoglobulin A2, Human Myeloma Plasma 16-16-090701-2M
Immunoglobulin D, Human Myeloma Plasma 16-16-090704-M
Immunoglobulin D, Human Plasma 16-16-090704
Immunoglobulin E, Human Myeloma Plasma 16-16-090705-M
Immunoglobulin E, Human Plasma 16-16-090705
Immunoglobulin G, Dog Plasma 16-16-090707-dog
Immunoglobulin G, Human Plasma 16-16-090707
Immunoglobulin G, Human Plasma, Fab Fragment 16-16-090707-Fab
Immunoglobulin G, Human Plasma, FC Fragment 16-16-090707-FC
Immunoglobulin G, Mouse Plasma 16-16-090707-mouse
Immunoglobulin G, Rat Serum 16-16-090707-rat
Immunoglobulin G1, Human Myeloma Plasma 16-16-090707-1M
Immunoglobulin G2, Human Myeloma Plasma 16-16-090707-2M
Immunoglobulin G3, Human Plasma 16-16-090707-3
Immunoglobulin G4, Human Myeloma Plasma 16-16-090707-4M
Immunoglobulin M, Fc5mu, Human Plasma 16-16-090713-Mfc5u
Immunoglobulin M, Human Myeloma Plasma 16-16-090713-M
Immunoglobulin M, Human Plasma 16-16-090713
Immunoglobulin M, mu Chain, Human Plasma 16-16-090713-MU
Kallikrein, Human Plasma 16-16-110112
Kininogen, HMW, Human Plasma 16-16-110914-H
Kininogen, LMW, Human Plasma 16-16-110914-L
Lactalbumin, Human Milk 13-16-011202
Lactoferrin, Human Neutrophil 16-14-120103
Lipoprotein a, [Lp(a)], Human Plasma 12-16-121601
Lipoproteins, High Density, Human Plasma 12-16-080412
Lipoproteins, High Density, Human Plasma, 适于细胞培养 12-16-080412-TC
Lipoproteins, Intermediate Density, Human Plasma 12-16-090412
Lipoproteins, Low Density, Human Plasma 12-16-120412
Lipoproteins, Low Density, Human Plasma, 适于细胞培养 12-16-120412-TC
Lipoproteins, Very Low Density, Human Plasma 12-16-221204
Lipoproteins, Very Low Density, Human Plasma, 适于细胞培养 12-16-221204-TC
Lysozyme, Human Neutrophil 16-14-122519
Myeloperoxidase, Human Neutrophil 16-14-130000
Plasmin, Human Plasma, Frozen 16-16-161213-F
Plasmin, Human Plasma, Lyophilized 16-16-161213-L
Plasminogen, Human Plasma 16-16-161200
Platelet Factor 4, Human Platelet 16-20-060306
Prealbumin, Human Plasma (Transthyretin) 16-16-161801
Proteinase 3, Human Neutrophil 16-14-161820
S-100 Protein, Bovine Brain 16-BR-190816-bovine
S-100b Protein, Bovine Brain 16-BR-190802-bovine
S-100αα Protein, Bovine Brain 16-BR-190801-bovine
Serum Amyloid A, Human Plasma 16-16-190101
Thrombospondin, Human Platelet 16-20-201319
Transferrin, Dog Plasma 16-16-032001-dog
Transferrin, Human Plasma 16-16-032001
Transferrin, Human Plasma, 组织培养级 16-16-032001-LEL
Transferrin, Mouse Plasma 16-16-032001-mouse
Transferrin, Rat Serum 16-16-032001-rat
Trypsin, Human Pancreas 16-19-032000
Vitronectin, Human Plasma 16-16-220920

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